Platelet-Derived Growth Factor-BB human P3201 from MilliporeSigma

Product Specs
ItemPlatelet-Derived Growth Factor-BB human
CompanyMilliporeSigma
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberP3201
Molecular Weight~25 kDa
Specieshuman
Molecule NameADRA1D, ADRB3, ADRB2
NCBI Full Gene Nameadrenoceptor beta 3, adrenoceptor beta 2, adrenoceptor alpha 1D
NCBI Gene AliasesADRA1A, B2AR, ALPHA1, BAR, BETA2AR, ADRA1, ADRB2R, ADRBR, ADRA1R, DAR, dJ779E11.2, BETA3AR
Applicationsmammalian cell culture
FormatLyophilized from a 0.2 µm filtered solution in 30 mM acetic acid
Quantity10µG, 50µG

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MilliporeSigma

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Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) pYtags enable spatiotemporal measurements of receptor tyrosine kinase signaling in living cellseLifeMay 22, 2023Farahani PE, Yang X, Mesev EV, Fomby KA, Brumbaugh-Reed EH, Bashor CJ, Nelson CM, Toettcher JE.protein",FGF4,R&D Systems,Cat # 235-F4-025, "Peptide, recombinant protein",PDGF-BB,Millipore Sigma,Cat # P3201, "Peptide, recombinant protein",VEGF-C,R&D Systems,Cat # 9199-VC-025, "Chemical compound, drug",Gefitinib,Cell Signaling Technology

(2) Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosisResearch SquareJanuary 21, 2023Hideaki Matsubayashi, Jack Mountain, Tony Yao, Amy Peterson, Abhijit Deb Roy, Takanari Inouefrozen fibronectin was thawed, the remainder was kept at 4°C. PDGF-BB was purchased from Sigma-Aldrich (P3201), reconstituted with 4 mM HCl containing 0.1% BSA to obtain 50 μg/mL stock solution, and stored at −20°C.

(3) Non-catalytic role of phosphoinositide 3-kinase in mesenchymal cell migration through non-canonical induction of p85β/AP-2-mediated endocytosisbioRxivJanuary 4, 2023Hideaki T. Matsubayashi, Jack Mountain, Tony Yao, Amy F. Peterson, Abhijit Deb Deb Roy, Takanari Inouefrozen fibronectin was thawed, the remainder was kept at 4°C. PDGF-BB was purchased from Sigma-Aldrich (P3201), reconstituted with 4 mM HCl containing 0.1% BSA to obtain 50 μg/mL stock solution, and stored at −20°C.

(4) Rheb regulates nuclear mTORC1 activity independent of farnesylationCell Chemical BiologyJune 1, 2022Yanghao Zhong, Xin Zhou, Kun-Liang Guan, Jin ZhangSan Diego respectively to perform experiments. METHOD DETAILS Reagents and Drug Treatment PDGF (P3201) was purchased from Sigma and was used at 50 ng/mL to stimulate starved cells. Rapamycin (R-5000) was

(5) Abi1 mediates airway smooth muscle cell proliferation and airway remodeling via Jak2/STAT3 signalingiScienceFebruary 18, 2022Wang R, Wang Y, Liao G, Chen B, Panettieri RA Jr, Penn RB, Tang DD.Ham’s F-12 nutrient mix,GIBCO,11765-047/2187149 Protein A/G Plus-Agarose,Santa Cruz Biotech.,SC-2003 PDGF-BB,Sigma,# P3201-10UG Lot# SLBW2507 & Lot# SLBZ6214 SDS,Bio-Rad,161-0302 Human STAT3,Abcam,Ab268982/GR3352364-3 Trypan

(6) New insights into the clinical and molecular spectrum of the novel CYFIP2-related neurodevelopmental disorder and impairment of the WRC-mediated actin dynamicsGenetics in MedicineMarch 1, 2021Anaïs Begemann et al.and incubated in medium supplemented with 10 pg/ml of human platelet derived growth factor BB (PDGF) (P3201, Sigma-Aldrich, St. Louis, Missouri). Migration was assessed by multiframe live cell imaging at 37 °C

(7) Identification of a Dexamethasone Mediated Radioprotection Mechanism Reveals New Therapeutic Vulnerabilities in GlioblastomaCancersJanuary 19, 2021Aldaz P, Auzmendi-Iriarte J, Durántez M, Lasheras-Otero I, Carrasco-Garcia E, Zelaya MV, Bragado L, Olías-Arjona A, Egaña L, Samprón N, et al.doses ranging from 6−12 Grays. Dexamethasone (cat#D4920), temozolomide (cat#T2577), human PDGF-BB (cat#P3201) and LPS (cat# L5293) were from Sigma/Merck (Madrid, Spain). IFNγ (cat#300-02) was from Peprotech (London,

(8) Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleusNature communicationsNovember 30, 2020Zhou X, Zhong Y, Molinar-Inglis O, Kunkel MT, Chen M, Sun T, Zhang J, Shyy JY, Trejo J, Newton AC, et al.made available by direct request from the investigators, as well as through Addgene. Reagents PDGF (P3201), insulin (I-5523), IBMX (I5879) and Gö6983 (G1918) were purchased from Sigma. Nuclear-ID Red (Enzo-52406)

(9) Enhancer of Zeste Homologue 2 Inhibition Attenuates TGF-β Dependent Hepatic Stellate Cell Activation and Liver FibrosisCellular and molecular gastroenterology and hepatologyJanuary 1, 2019Martin-Mateos R, De Assuncao TM, Arab JP, Jalan-Sakrikar N, Yaqoob U, Greuter T, Verma VK, Mathison AJ, Cao S, Lomberk G, et al.Houston, TX) (10 mmol/L) and/or TGF-β (240-B; R&D Systems, Minneapolis, MN) (10 ng/mL) and/or PDGF-BB (P3201; Sigma-Aldrich) (10 ng/mL) for 48 hours. All cell lines were maintained under standard tissue culture

(10) Platelet-Derived Growth Factor Subunit B Signaling Promotes Pericyte Migration in Response to Loud Sound in the Cochlear Stria VascularisJournal of the Association for Research in Otolaryngology : JAROAugust 1, 2018Hou Z, Wang X, Cai J, Zhang J, Hassan A, Auer M, Shi X.Cell Migration Assay, Cell Biolabs, Inc., San Diego, CA, USA) containing PDGF-BB at 2, 4, or 8 ng/ml (P3201, Sigma-Aldrich, USA). The Boyden chambers were placed in an incubator for 6 h. Migrated pericytes passed

(11) De Novo Truncating Mutations in WASF1 Cause Intellectual Disability with SeizuresThe American Journal of Human GeneticsJuly 1, 2018Yoko Ito, Keren J. Carss, Sofia T. Duarte, Taila Hartley, Boris Keren, Manju A. Kurian, Isabelle Marey, Perinne Charles, Carla MendonÃ§a, Caroline Nava, et al.poly-l-lysine-coated coverslips, and stimulated with platelet-derived growth factor (PDGF; Sigma-Aldrich, P3201) for inducing the formation of lamellipodia, as previously described.20 Then cells were fixed, filamentous

(12) Baicalin inhibits PDGF-BB-induced hepatic stellate cell proliferation, apoptosis, invasion, migration and activation via the miR-3595/ACSL4 axisInternational journal of molecular medicineApril 1, 2018Wu X, Zhi F, Lun W, Deng Q, Zhang W.were serum-starved in an FBS-free medium for 24 h and then induced with PDGF-BB (10 ng/ml; cat. no. P3201; Sigma-Aldrich; Merck KGaA, Darmstadt, Germany), following the protocol in a previous study (26). The

(13) Matrix stiffness regulates migration of human lung fibroblastsPhysiological reportsMay 1, 2017Asano S, Ito S, Takahashi K, Furuya K, Kondo M, Sokabe M, Hasegawa Y.400 μL DMEM/F‐12 containing 0.1% FBS (2 × 105 cells/mL), and placed in the upper chamber. PDGF‐BB (P3201; Sigma‐Aldrich) prepared in 0.1% BSA in phosphate‐buffered saline (PBS) dissolved in DMEM/F‐12 containing

(14) Heparan sulfate 6-O-endosulfatases, Sulf1 and Sulf2, regulate glomerular integrity by modulating growth factor signalingAmerican Journal of Physiology-Renal PhysiologyMarch 1, 2016Yasutoshi Takashima, Kazuko Keino-Masu, Hiroshi Yashiro, Satoshi Hara, Tomo Suzuki, Toin H. van Kuppevelt, Masayuki Masu, Michio Nagata(Sigma-Aldrich), subsequently incubated with RPMI-1640 supplemented with 10% FBS that contained 10 ng/ml PDGF-B (P3201, Sigma-Aldrich), 50 ng/ml VEGF (PMG0111, Biosource), 10 ng/ml transforming GF (TGF)-␤ (240-B/CF, R&D

(15) PDGF beta targeting in cervical cancer cells suggest a fine-tuning of compensatory signalling pathways to sustain tumourigenic stimulationJournal of Cellular and Molecular MedicineFebruary 1, 2015Oana Mihaela Tudoran et al.BSA solution for 1 hr and incubated with 100 μl of cell media or serial dilutions of PDGFBB protein (P3201-10UG; Sigma-Aldrich) for 2 hrs followed by 1 hr incubation with 100 μl of 0.5 μg/ml PDGFBB detection

(16) Brivanib attenuates hepatic fibrosis in vivo and stellate cell activation in vitro by inhibition of FGF, VEGF and PDGF signalingPloS oneJanuary 1, 2014Nakamura I, Zakharia K, Banini BA, Mikhail DS, Kim TH, Yang JD, Moser CD, Shaleh HM, Thornburgh SR, Walters I, et al.used. To study the effect of brivanib on growth factor signaling pathways, human recombinant PDGF-BB (P3201, Sigma-Aldrich, St. Louis, MO, USA), VEGF, FGF and TGF-β1 (293-VE, 233-FB and 5036-WN, respectively,

(17) Micropatterning Different Cell Types with Microarray Amplification of Natural Directional PersistenceAdvanced Healthcare MaterialsFebruary 1, 2013Kyu-Shik Mun, Girish Kumar, Carlos C. Co, Chia-Chi HoMedia (EGM; Catalog No. CC-3162) was from Lonza. Platelet Derived Growth Factor (PDGF; Catalog No. P3201) and Bovine Serum Albumin (BSA; Catalog No. A7906) were from Sigma-Aldrich. Alexa fluor 488 Phalloidin

(18) P-Rex1 cooperates with PDGFRβ to drive cellular migration in 3D microenvironmentsPloS oneJanuary 1, 2013Campbell AD, Lawn S, McGarry LC, Welch HC, Ozanne BW, Norman JC.Bioscience. Recombinant human EGF (AF-100-15) was obtained from Peprotech EC Ltd, recombinant human PDGF-BB (P3201), and NRG1 (SRP3055) were obtained from Sigma-Aldrich Ltd. siRNA Treatments Transient knockdown of

(19) Islet grafting and imaging in a bioengineered intramuscular space†TransplantationNovember 15, 2009Piotr Witkowski et al.recombinant human VEGF165 (PHG0145, Invitrogen, Carlsbad, USA) and 100 ng/ml recombinant human PDGFbb (P3201, Sigma Aldrich, St Louis, USA) to cRGDfK alginate, calcium gluconate and sterile distilled water solutions. On

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Platelet-Derived Growth Factor-BB human from MilliporeSigma

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Platelet-Derived Growth Factor-BB human from MilliporeSigma

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